CHALLENGE TEST IN COSMETICS: WHAT IT IS AND WHY IT ENSURES PRODUCT PRESERVATION

Dr. Elena Domeniconi, Head of the Microbiology Laboratory

The Challenge Test is a fundamental microbiological test used to evaluate the effectiveness of the preservative system in cosmetics. Its main purpose is to ensure that the product remains safe, stable, and free from contamination throughout its entire period of use by the consumer.

How Does the Challenge Test Work?

During the test, the cosmetic product is deliberately contaminated in a laboratory with a mixture of five selected microorganisms: Gram-positive bacteria, Gram-negative bacteria, yeasts, and filamentous fungi.
The goal is to simulate real-life conditions under which the product may come into contact with external agents during production, storage, or daily use.

Microbial activity is monitored over a 28-day period to assess the preservative's long-term efficacy.

When Is the Challenge Test Mandatory?

The Challenge Test is mandatory for all cosmetic products that contain water-based or natural ingredients, such as:

• emulsions (creams, lotions),

• products with botanical extracts,

• formulas without artificial preservatives.

These types of cosmetics are more prone to microbial growth and therefore require a thorough evaluation of their preservation system.

Benefits of the Challenge Test for the Consumer

Thanks to the Challenge Test:

• the safety of the cosmetic product is ensured,

• the effectiveness of active ingredients is preserved over time,

• the health of the final consumer is protected.

In Summary

The Challenge Test is a cornerstone of modern cosmetology. Performing this test is not only a regulatory requirement, but also a responsible practice that guarantees reliable, safe, and high-performing products until the very last use.

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